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SIRT4 targets identified by Human Protein <t>Microarray.</t> A) Scan of Human Protein Microarray in the presence or absence (Control) of human recombinant SIRT4 protein. The pattern of red fluorescence spots (always in duplicate) on the control chip serves to facilitate specific protein identification following fluorescence excitation. Fluorescence spots specifically identified on the array incubated with SIRT4 represent potential SIRT4 interaction targets (exemplarily illustrated by the white arrow). B) List of proteins identified by protein-protein interaction with SIRT4 using a Human Protein Microarray. SIRT4, sirtuin 4. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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SIRT4 targets identified by Human Protein Microarray. A) Scan of Human Protein Microarray in the presence or absence (Control) of human recombinant SIRT4 protein. The pattern of red fluorescence spots (always in duplicate) on the control chip serves to facilitate specific protein identification following fluorescence excitation. Fluorescence spots specifically identified on the array incubated with SIRT4 represent potential SIRT4 interaction targets (exemplarily illustrated by the white arrow). B) List of proteins identified by protein-protein interaction with SIRT4 using a Human Protein Microarray. SIRT4, sirtuin 4. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: Journal of Molecular and Cellular Cardiology Plus

Article Title: Sirtuin 4 accelerates heart failure development by enhancing reactive oxygen species-mediated profibrotic transcriptional signaling

doi: 10.1016/j.jmccpl.2025.100299

Figure Lengend Snippet: SIRT4 targets identified by Human Protein Microarray. A) Scan of Human Protein Microarray in the presence or absence (Control) of human recombinant SIRT4 protein. The pattern of red fluorescence spots (always in duplicate) on the control chip serves to facilitate specific protein identification following fluorescence excitation. Fluorescence spots specifically identified on the array incubated with SIRT4 represent potential SIRT4 interaction targets (exemplarily illustrated by the white arrow). B) List of proteins identified by protein-protein interaction with SIRT4 using a Human Protein Microarray. SIRT4, sirtuin 4. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: SIRT4 protein interaction was investigated using a Human Protein Microarray (Catalog # 27055101, Thermo Fisher Scientific, Germany) following the manufacturing protocol.

Techniques: Microarray, Control, Recombinant, Fluorescence, Incubation